HaloTag8: an engineered protein tag improving fluorophore performance and enabling fluorescence lifetime multiplexing.
- Date: May 6, 2021
- Time: 03:00 PM (Local Time Germany)
- Speaker: Michelle Frei
- present position: Max-Planck-Institute for Medical Research in Heidelberg
- Location: web-talk
- Room: Zoom-Meeting
- Host: Max Planck BioImaging Core Unit Network - Spotlight Talks
Meeting ID: 894 9575 9133 Passcode: 579505
Michelle Frei will talk about the following preprint:
HaloTag8: an engineered protein tag to improve fluorophore performance
Abstract
HaloTag8 is an engineered variant of HaloTag7 with up to 40% higher
brightness and increased fluorescence lifetime when labeled with
fluorogenic rhodamines. Moreover, combining HaloTag8 with HaloTag7 and
other fluorescent probes enabled live-cell multiplexing using a single
fluorophore and the generation of a fluorescence lifetime-based
biosensor. The increased brightness of HaloTag8 and its use in
fluorescence lifetime multiplexing makes it a powerful tool for
live-cell imaging.