Regional specialization and fate specification of bone stromal cells in skeletal development
- Date: Oct 21, 2021
- Time: 03:00 PM - 04:15 PM (Local Time Germany)
- Location: web-talk
- Room: Zoom-Meeting
- Host: Max Planck BioImaging Core Unit Network - Spotlight Talks
Dr. Kishor Kumar Sivaraj will talk about the following paper:
Regional specialization and fate specification of bone stromal cells in skeletal development
Abstract
Bone stroma contributes to the regulation of osteogenesis and
hematopoiesis but also to fracture healing and disease processes.
Mesenchymal stromal cells from bone (BMSCs) represent a heterogenous
mixture of different subpopulations with distinct molecular and
functional properties. The lineage relationship between BMSC subsets and
their regulation by intrinsic and extrinsic factors are not well
understood. Here, we show with mouse genetics, ex vivo cell
differentiation assays, and transcriptional profiling that BMSCs from
metaphysis (mpMSCs) and diaphysis (dpMSCs) are fundamentally distinct.
Fate-tracking experiments and single-cell RNA sequencing indicate that
bone-forming osteoblast lineage cells and dpMSCs, including leptin
receptor-positive (LepR+) reticular cells in bone marrow,
emerge from mpMSCs in the postnatal metaphysis. Finally, we show that
BMSC fate is controlled by platelet-derived growth factor receptor β
(PDGFRβ) signaling and the transcription factor Jun-B. The sum of our
findings improves our understanding of BMSC development, lineage
relationships, and differentiation.